crossflow ultrafiltration (Sartorius AG)

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Sartorius AG crossflow ultrafiltration
Crossflow Ultrafiltration, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 98/100, based on 881 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crossflow+ultrafiltration/Vivaspin+100+PES+Centrifugal+Concentrator/pm40362319-300-5-12
Average 98 stars, based on 881 article reviews

crossflow ultrafiltration - by Bioz Stars, 2026-11

98/100 stars

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Filtration:

Article Title: Proteomics of Bacterial and Mouse Extracellular Vesicles Released in the Gastrointestinal Tracts of Nutrient-Stressed Animals Reveals an Interplay Between Microbial Serine Proteases and Mammalian Serine Protease Inhibitors.
Article Snippet: The supernatants were then centrifuged at 5500× g and 4 ◦C for 15 min and filtered through polyethersulfone (PES) membranes (0.22 μm pore size) (Sartorius, Göttingen, Germany) to remove debris and remaining cells. .. Vesicle suspensions were concentrated by crossflow ultrafiltration (100 kDa MWCO, Vivaflow 200, Sartorius) to 5 mL, rinsed via the addition of 500 mL of PBS, pH 7.4, and concentrated again by crossflow filtration to 5 mL, and the retentates were concentrated to 1 mL with a Vivaspin 20 centrifugal concentrator (100 kDa molecular weight cut-off, Sartorius). .. Further purification of the mixture of BEVs and EVs was performed by size exclusion chromatography (SEC) using either CL2-B Sepharose (Sigma-Aldrich, St. Louis, MO, USA) in PBS buffer for the proteomics analysis, as previously described [21], or Int.

Article Title: Extracellular vesicles produced by the human commensal gut bacterium Bacteroides thetaiotaomicron affect host immune pathways in a cell‐type specific manner that are altered in inflammatory bowel disease
Article Snippet: .. BEVs in the 500 ml filtrates were concentrated by crossflow ultrafiltration (100 kDa MWCO, Vivaflow 50R, Sartorius) to 0.5 ml, diluted by addition of 500 ml of ice‐cold phosphate buffered saline (PBS), pH 7.4, and the suspensions were concentrated again by crossflow filtration to 0.5 ml and filter‐sterilised through a 0.22 μm PES membrane (Sartorius). .. Following crossflow ultrafiltration, further purification of BEVs was performed by fractionation of the suspension (Durant et al., ) by size‐exclusion chromatography using a CL2‐B Sepharose (Sigma‐Aldrich) (120 cm × 1 cm column) in PBS buffer.

Article Title: Proteomics of Bacterial and Mouse Extracellular Vesicles Released in the Gastrointestinal Tracts of Nutrient-Stressed Animals Reveals an Interplay Between Microbial Serine Proteases and Mammalian Serine Protease Inhibitors
Article Snippet: The supernatants were then centrifuged at 5500× g and 4 °C for 15 min and filtered through polyethersulfone (PES) membranes (0.22 μm pore size) (Sartorius, Göttingen, Germany) to remove debris and remaining cells. .. Vesicle suspensions were concentrated by crossflow ultrafiltration (100 kDa MWCO, Vivaflow 200, Sartorius) to 5 mL, rinsed via the addition of 500 mL of PBS, pH 7.4, and concentrated again by crossflow filtration to 5 mL, and the retentates were concentrated to 1 mL with a Vivaspin 20 centrifugal concentrator (100 kDa molecular weight cut-off, Sartorius). .. Further purification of the mixture of BEVs and EVs was performed by size exclusion chromatography (SEC) using either CL2-B Sepharose (Sigma-Aldrich, St. Louis, MO, USA) in PBS buffer for the proteomics analysis, as previously described [ ], or using a qEV/35 nm series SEC column according to the manufacturer’s instructions (IZON Science, Lyon, France), with the pooled collected fractions filtered through PES membranes (0.22 μm pore size) and stored at 4 °C.

Article Title: The Proteome of Extracellular Vesicles Produced by the Human Gut Bacteria Bacteroides thetaiotaomicron In Vivo Is Influenced by Environmental and Host-Derived Factors
Article Snippet: .. BEVs in the 500 mL filtrates were concentrated by crossflow ultrafiltration (100 kDa MWCO, Vivaflow 50R, Sartorius) to 2 mL, diluted by addition of 500 mL phosphate-buffered saline (PBS), pH 7.4, and the suspensions were concentrated again by crossflow filtration and filter-sterilized through a 0.22 μm PES membrane (Sartorius). .. Following crossflow ultrafiltration, further purification of BEVs was performed by fractionation of the suspension by size exclusion chromatography using a CL2-B Sepharose (Sigma-Aldrich) (120 cm x 1 cm column) in PBS buffer ( ) onto which 1 mL of BEV suspension was loaded.

Molecular Weight:

Article Title: Proteomics of Bacterial and Mouse Extracellular Vesicles Released in the Gastrointestinal Tracts of Nutrient-Stressed Animals Reveals an Interplay Between Microbial Serine Proteases and Mammalian Serine Protease Inhibitors
Article Snippet: The supernatants were then centrifuged at 5500× g and 4 °C for 15 min and filtered through polyethersulfone (PES) membranes (0.22 μm pore size) (Sartorius, Göttingen, Germany) to remove debris and remaining cells. .. Vesicle suspensions were concentrated by crossflow ultrafiltration (100 kDa MWCO, Vivaflow 200, Sartorius) to 5 mL, rinsed via the addition of 500 mL of PBS, pH 7.4, and concentrated again by crossflow filtration to 5 mL, and the retentates were concentrated to 1 mL with a Vivaspin 20 centrifugal concentrator (100 kDa molecular weight cut-off, Sartorius). .. Further purification of the mixture of BEVs and EVs was performed by size exclusion chromatography (SEC) using either CL2-B Sepharose (Sigma-Aldrich, St. Louis, MO, USA) in PBS buffer for the proteomics analysis, as previously described [ ], or using a qEV/35 nm series SEC column according to the manufacturer’s instructions (IZON Science, Lyon, France), with the pooled collected fractions filtered through PES membranes (0.22 μm pore size) and stored at 4 °C.

Saline:

Membrane:

Article Title: Unassembled cell wall proteins form aggregates in the extracellular space of Chlamydomonas reinhardtii strain UVM4
Article Snippet: C. reinhardtii cells and medium were separated by tangential flow microfiltration with a 0.2 μm polyethersulfone (PES) membrane (Vivaflow 200, Sartorius, Göttingen, Germany) at the lowest speed. .. The filtrate (growth medium) was subsequently concentrated 250-fold by crossflow ultrafiltration with a 3 kDa cutoff (3000 MWCO PES membrane, Vivaflow 200 and Vivaspin Turbo 15, Sartorius, Göttingen, Germany). ..

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